![]() ![]() Despite this limitation, the nucleic acid amplification method has the potential to be used for differentiating the species and strains of isolates and clinical specimens. tuberculosis in clinical samples is generally less sensitive than the detection of other pathogens due to the relatively low numbers of bacilli present. However, because this method is limited by the expensive equipment required, the Gene Xpert assay is still rarely used in countries with a high HIV/TB prevalence. The accuracy of the Gene Xpert test offers a significant increase in terms of diagnostic sensitivity, even when it is deployed selectively, i.e., among only smear-negative presumptive TB patients. The significant advance in the diagnosis of TB in the last decade has been the advent of the Gene Xpert MTB/RIF (Gene Xpert) test. tuberculosis is more sensitive and rapid than solid culture but is prone to contamination in some laboratories, so it is best to use both methods in conjunction. ![]() Solid culture is usually performed on Lowenstein–Jensen (LJ), Ogawa, or Middlebrook 7H10/11. The conventional culture method for Mycobacterium tuberculosis is a more sensitive technique by which to detect TB compared with AFB smears, but, due to the slow growth of the organism, sputum cultures take 4–6 weeks to become positive on solid media and 10–21 days in liquid media. Previous work in Brazil on the accuracy of the AFB smear method reported that this test has a sensitivity of 36% and a specificity of 100%. Therefore, early TB diagnosis and management is critical.Īn acid-fast bacilli (AFB) smear is used to screen for new TB infection, and this method requires fresh sputum from patients as input samples. Transmission is rapidly reduced with effective treatment. Overall, the risk of infection among household contacts of TB patients is around 30%. Factors influencing the chance of transmission include the bacillary load of the source case, e.g., higher transmission occurs from active pulmonary cases who are sputum smear-positive or who display lung cavities on a chest radiograph, as well as the proximity and duration of exposure. The transmission of TB occurs by inhalation of infectious droplet nuclei containing viable bacilli (aerosol spread). TB usually appears several years after infection, often when the immune system function is decreased by some other cause. ![]() This group is composed of anaerobic, acid-fast, slow-growing bacteria, and it can be difficult to determine the species responsible for specific TB cases. TB infection is caused by members of the Mycobacterium tuberculosis Complex (MTBC), which is the major group responsible for human pulmonary infections. To combat this, a timely diagnosis and the application of appropriate treatment are required. The number of TB deaths remains unacceptably high. Tuberculosis (TB) is a major global health problem this disease has caused more deaths than any other infectious disease over the last 200 years. tuberculosis-negative using the culture method, this method yielded the same results as the Gene Xpert test. Additionally, nucleic acid amplification also detected positive results among samples assessed as M. Some samples that yielded negative results in the AFB smear test were detected as positive for Mycobacterium tuberculosis using the nucleic acid amplification test, with a sensitivity of 83.1%. This study used clinical samples from tuberculosis patients and assessed them using a series of tests, aiming to increase the sensitivity of active tuberculosis detection. The purpose of this study was to assess different tuberculosis detection methods as part of serial tests and determine the best diagnostic approach for detecting active lung tuberculosis in Indonesia. The genus Mycobacterium has many species, strains, and variants, and their natural differences may affect the clinical outcome of the diseases they induce. In accordance with the recommendation of the World Health Organization, the Indonesian Tuberculosis Control Program uses the acid fast bacilli (AFB) smear and Chest X-ray methods as the primary methods for detecting tuberculosis, especially in new cases of suspected tuberculosis. Rapid detection and accurate diagnosis are very important in managing active tuberculosis because they provide an advantage in preventing further disease transmission. ![]()
0 Comments
Leave a Reply. |